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Yong Xu, Speaker at Chemical Engineering Conferences
Tongji University School of Medicine, China
Title : Nanofibrillar decellularized Wharton’s Jelly Matrix for segmental tracheal repair

Abstract:

Wharton’s jelly (WJ) is considered a potential scaffold in tissue-engineered trachea for its similar composition and function to cartilage tissue. However, the feasibility of using WJ to construct engineered neocartilage tissue has not been reported, let alone tubular tracheal cartilage regeneration and segmental tracheal lesion repair. Here, electrospun nanofibrous membranes composed of three different decellularized WJ matrix (DWJM)/poly(ε-caprolactone) (PCL) ratios (8:2, 5:5, and 2:8) are fabricated. The results demonstrate improved degradation speed, absorption, and cell adhesion capacity but weakened mechanical properties with increased DWJM content, but satisfactory homogeneous cartilage regeneration is only achieved in the DWJM/PCL (8:2) group after 12 weeks in vivo culture. Furthermore, homogeneous, 3D, tubular, trachea-shaped cartilage is constructed with a controllable lumen diameter and wall thickness based on the 2D nanofibrous membrane using a modified sandwich model, in which the chondrocyte-membrane construct is rolled around a silicon tube. Most importantly, by combining the above schemes with previously established vascularization and epithelialization techniques, chondrification, vascularization, and epithelialization are achieved simultaneously thus realizing long-term (6 months) circumferential tracheal lesion repair in a rabbit model with a biological structure and function similar to that of native trachea, representing a promising approach for the clinical application of tracheal tissue engineering.

Audience take-away:

  • Wharton’s jelly (WJ) is a suitable native-derived scaffold for cartilage tissue engineering.
  • Electrospun nanofibrous membranes composed of three different decellularized WJ matrix (DWJM)/poly(ε-caprolactone) (PCL) could be used for tracheal regeneration.
  • By combining the engineered trachea with previously established vascularization and epithelialization techniques, chondrification, vascularization, and epithelialization are achieved simultaneously thus realizing long-term (6 months) circumferential tracheal lesion repair in a rabbit model with a biological structure and function similar to that of native trachea.

Biography:

Yong Xu received his MCs degree from the Department of Thoracic Surgery, Shanghai Pulmonary Hospital, Tongji University School of Medicine in 2018. Now he is continuing to study for a PhD degree in the Department of Thoracic Surgery, Shanghai Pulmonary Hospital, Tongji University School of Medicine. His research interests focus on cartilage regenerative biomaterials and functional tracheal reconstruction.

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