In addition to other applications where great selectivity and benign reaction conditions are required, biocatalysis has evolved into a mature technology. As an alternative to chemical catalysis, biocatalysis has been used extensively in many different disciplines. The most well-known examples include enzymes' usage in organic synthesis, particularly when creating chiral chemicals for medicines and the flavour and fragrance sector. Additionally, biocatalysts are widely employed to produce speciality and even bulk compounds. Over the past two decades, biocatalysis has become a relatively established and commonly utilised technique. With a few notable exceptions, biocatalysis in the early 2000s continued to operate in specialised fields and concentrated on the production or removal of optically active intermediates. Modern bioinformatics and computer-supported enzyme engineering are significant driving forces in the quick identification of novel enzyme variations. Despite the fact that enzymes have incredibly high catalytic activity, their stability and cost are frequently seen as limitations.