Bond cleavage occasionally necessitates the use of catalysts. A significant amount of energy is needed to separate the hydrogen atom from the carbon and bond another atom to the carbon since C-H bonds have a high bond-dissociation energy of roughly 100 kcal/mol (420 kJ/mol). Catabolism is the breakdown of big molecules in biochemistry by severing their internal links. Unless they catalyse hydrolysis or oxidoreduction instead, in which case they are referred to as hydrolases and oxidoreductases, respectively, enzymes that catalyse bond cleavage are known as lyases. In proteomics, cleaving agents are employed in proteome analysis, when proteins are broken down into smaller peptide fragments. Cyanogen bromide, pepsin, and trypsin are a few examples of cleaving agents that are employed. The bond breaks in a process known as heterolytic cleavage, also One of the pieces is left with the shared pair of electrons at the beginning of the bond's breakdown. As a result, one fragment acquires an electron while the other fragment loses an electron, despite possessing both bonding electrons. Ionic fission is the alternative name for this process. The energy needed for heterolytic dissociation is the singlet excitation energy of a sigma bond, although because to the Coulombic interaction between the two ion fragments, the actual singlet excitation energy could be lower than the bond dissociation energy. Despite having bond strengths of 80 kJ/mol and 70 kJ/mol, respectively, a silicon-silicon sigma bond's singlet excitation energy is lower than that of a carbon-carbon sigma bond due to silicon's stronger electron affinity and lower ionisation potential than carbon.